Figure 1

Fitness of ancestral and selected phage on various tryptophan analogues. Ancestral and round 25 selected phage were tested for fitness on eight additional tryptophan analogues (95% analogue, 5% W) for Line 1 (left) and Line 2 (right). Analogues used were 4-fluorotryptophan (4fW), 5-fluorotryptophan (5fW), 4-methyltryptophan (4MeW), 5-methyltryptophan (5MeW), 6-methyltryptophan (6MeW), 7-methyltryptophan (7MeW), 5-hydroxytryptophan (5OHW) and 5-methoxytryptophan (5MeOW). Data for fitness on 95% 6fW and W are taken from Figures 3 and 5 respectively. Error bars represent standard deviations of at least three replicates.