Table 5 Essential genes missing in LUCA0.9 and their appearance in versions of LUCA with greater g-valuesa
Gene name | COG no. | Minimal g-value | Function |
|---|---|---|---|
TRANSLATION AND RIBOSOME BIOGENESIS | |||
GatC | COG0721 | 1.0 | Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit |
LysU | COG1190 | 1.0 | Lysyl-tRNA synthetase |
GRS1 | COG0423 | 1.5 | Glycyl-tRNA synthetase |
CENTRAL METABOLIC PATHWAYS AND SUGAR METABOLISM | |||
Glycolysis | |||
PfkA | COG0205 | 1.0 | 6-phosphofructokinase |
Fba | COG0191 | 1.0 | Fructose bisphosphate aldolase |
GpsA | COG0240 | 1.0 | Glycerol 3-phosphate dehydrogenase |
GpmA | COG0588 | 2 | Phosphoglycerate mutase, cofactor-dependent |
TCA cycle | Â | Â | |
AcnA | COG1048 | 1.0 | Aconitase A |
PorG | COG1014 | 1.0 | Pyruvate:ferredoxin oxidoreductase |
AceF | COG0508 | 1.0 | Dihydrolipoamide acyltransferases |
FumC | COG0114 | 1.0 | Fumarase |
TtdA | COG1951 | 3.0 | Fumarate hydratase class I, N-terminal domain |
FumA | COG1838 | 3.0 | Fumarate hydratase class I, C-terminal domain |
Pentose phosphate shunt | |||
Zwf | COG0364 | 1.0 | Glucose-6-phosphate 1-dehydrogenase |
NagB | COG0363 | 1.0 | 6-phosphogluconolactonase/Glucosamine-6- |
MipB | COG0176 | 1.0 | Transaldolase |
Rpe | COG0036 | 1.0 | Pentose-5-phosphate-3-epimerase |
RpiA | COG0120 | 1.0 | Ribose 5-phosphate isomerase |
Gnd | COG0362 | 1.5 | 6-phosphogluconate dehydrogenase |
NUCLEOTIDE METABOLISM | |||
Adk | COG0563 | 1.0 | Adenylate kinase and related kinases |
Cdd | COG0295 | 1.0 | Cytidine deaminase |
- | COG0590 | 1.0 | Cytosine/adenosine deaminase |
DeoC | COG0274 | 1.0 | Deoxyribose-phosphate aldolase |
Gmk | COG0194 | 1.0 | Guanylate kinase |
PurS | COG1828 | 1.0 | Phosphoribosylformylglycinamidine (FGAM) |
THY1 (ThyX) | COG1351 | 1.0 | Predicted alternative thymidylate synthase |
Pnp | COG0005 | 1.0 | Purine nucleoside phosphorylase |
PyrH | COG0528 | 1.0 | Uridylate kinase |
Cmk | COG1102 | >3 | Cytidylate kinase |
Tdk | COG1435 | 1.5 | Thymidine kinase |
AMINO ACID METABOLISM | |||
AroG | COG0722 | 3.0 | 3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase |
AroA | COG2876 | 2.0 | 3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase |
AroD | COG0710 | 1.5 | 3-dehydroquinate dehydratase |
AroQ | COG0757 | 1.5 | 3-dehydroquinate dehydratase II |
AroK | COG0703 | 1.0 | Shikimate kinase |
TyrB | COG1448 | 3.0 | Aspartate/aromatic aminotransferase |
HIS2 | COG1387 | 1.0 | Histidinol phosphatase and related PHP family phosphatases |