Table 7 Sequence of primers/adaptors used for ITS, AFLP and SSRs

5′-CCTTATCATTTAGAGGAAGGAG-3′

ITS5a

5′-TCCTCCGCTTATTGATATGC-3′

ITS4

Sequence of adaptors and primers used for AFLP

5′-CTCGTAGACTGCGTACC-3′

EcoRI adaptors

5′-AATTGGTACGCAGTCTAC-3′

5′-GACGATGAGTCCTGAG-3′

MseI adaptors

5′-TACTCAGGACTCAT-3′

5′-GACTGCGTACCAATTCA-3′ (EcoRI + A)

Pre-selective primers

5′-GATGAGTCCTGAGTAAA-3′ (MseI + A)

EcoRI + ACA/MseI + ATC (set1)

Final amplification

EcoRI + ACC/MseI + ATC (set2)

EcoRI + ACA/MseI + ACA (set3)

EcoRI + ACC/MseI + ACA (set4)

Sequence of primers used for SSRs

5′-GAATGGGAAGGTGAAAGAG-3′

HO5

5′-CACGGCACTGTTCATCTAC-3′

5′-ATAACCAAAGCCTCCCAAGC-3′

HO8

5′-AAATATCAAACGCCCCTCAC-3′

5′-CAACTAACCAAACGAGAAAC-3′

HO36

5′-AACCTTGACACCTGCTAATA-3′

5′-ATCGAACCCAATAGACACCAG-3′

HO48

5′-CAGGCAACTTAGCAAGAAACT-3′

5′-AGATAAGTTTCACTCCTGTG-3′

HO51

5′-ACCAGAACCAATCAAGAT-3′