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Table 2 Primer pairs used for PCR amplification and Sanger sequencing

From: Clonality, recombination, and hybridization in the plumbing-inhabiting human pathogen Fusarium keratoplasticuminferred from multilocus sequence typing

Locus

Fw primer

Rv primer

Annealing temp. (°C)

Size1(bp)

Seq. composition (bp)2

Alleles3

H 4

TEF

EF1: ATGGGTAAGGARGACAAGAC

EF2: GGARGTACCAGTSATCATGTT

53

667

256 intronic; 411 exonic

22

0.89

rDNA

ITS5: GGAAGTAAAAGTCGTAACAAGG

NL4: GGTCCGTGTTTCAAGACGG

53

1029

705 coding; 324 ITS

6

0.35

RPB2 5-7

5f2: GGGGWGAYCAGAAGAAGGC

7cr: CCCATRGCTTGYTTRCCCAT

55

863

Exonic

11

0.51

RPB2 7-11

7cf: ATGGGYAARCAAGCYATGGG

11ar: GCRTGGATCTTRTCRTCSACC

55

881

Exonic

-

-

3968

3968fw: TGTTGGTTCGAGATGGTTGA

3968rv: GAGAAGGGCAACTGGGAGA

53

770

Intergenic

12

0.68

3972

3972fw: TCTGATGCAGACTAGCACTCG

3972rv: ATCGGACGAAACAGAGCAGG

53

831

Intergenic

13

0.45

4081

4081fw: TGACRAGGATGAATGAGCGA

4081rv: TGACCAGCCTCCAAGSG

56

642

Intergenic

18

0.8

6512

6512fw: GGAGGACCAGGAGGAATAGG

6512rv: CAAAGCAGATCGACTGAGGA

53

644

Intergenic

12

0.52

5439

5439fw: AATGGGAATACGAGCGTCAG

5439rv: AGGGGCTGCTGTTAGTGAGA

53

779

Intergenic

10

0.48

5437

5437fw: AACAAGACAAGGCAGCAGGT

5437rv: TCCAGAGGAACGACGAGGC

56

544

213 exonic, 331 intergenic

23

0.88

MAT1-1

MAT1-S-1 F: ATGGCTTTCCGCAGTAAGGA

MAT1-S-1R: CATGATAGGGCAGCAAAGAG

53

~200

N. A.

N. A.

N. A.

MAT1-2

MAT2-S-1 F: GGGAATCTGAGAAAGATACGTAC

MAT2-S-1R: CGGTACTGGTAGTCGGGAT

53

~800

N. A.

N. A.

N. A.

  1. 1. Number of characters in the aligned dataset. For different isolates, the exact length of any region may be variable due to indels and microsatellite repeats 2. ITS = internal transcribed spacers 1 and 2. Locus 5437 contains coding regions of a predicted hypothetical protein of unknown function. 3. Number of alleles observed in F. keratoplasticum 4. Average genetic diversity of each of the 9 loci calculated using LIAN accessed at http://pubmlst.org.