Skip to main content

Table 3 The enzyme kinetic parameters of SpDJ-1, the predicted catalytically inactive mutants of SpDJ-1, Hsp3101, Hsp3102, ScHsp31 and ScHsp32

From: Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe

Enzyme

Km(mM)

kcat(min-1)

kcat/Km(min-1 M-1)

SpDJ-1

10.8

85.7

7.9 × 103

SpDJ-1E16A

ND

ND

ND

SpDJ-1C111A

ND

ND

ND

SpDJ-1H130A

37.3

65.5

1.8 × 103

Hsp3101

1.4

31.1

2.3 × 104

Hsp3102

2.7

58.0

2.1 × 104

ScHsp31

1.5

75.0

5.1 × 104

ScHsp32

ND

ND

ND

  1. All enzyme assays were done in triplicate, and the mean values are presented. ND, not determined. The kinetic parameters of SpDJ-1E16A, SpDJ-1C111A and ScHsp32 cannot be accurately measured because of the extremely low activity of these proteins.
Back to article page

BMC Ecology and Evolution

ISSN: 2730-7182

Contact us