Figure 5

Overexpression of hsp3101, 3102, ScHSP31 and EcHsp31 increases resistance of wild-type and Δ Spglo1 cells to α-dicarbonyls. (A-B) Wild-type cells harboring pREP3X (empty plasmid) or indicated constructs overexpressing Spglo1 (positive control), EcHsp31, ScHSP31, hsp3101-3103 and SpDJ-1. (C) Wild-type cells harboring pREP3X and ΔSpglo1 cells harboring indicated constructs. Cells were grown to stationary phase in leucine-selective medium. Equivalent numbers of cells, in 10-fold serial dilutions (from left to right), were spotted on leucine-selective media without or with the indicated concentration of either MG (A and C) or GO (B) and were incubated at 30°C.