Figure 1

Bayesian-inference phylogenetic tree of T. tubifex and T. blanchardi from the Lambro River, based on a combined 16S rRNA and COI genes data set, compared to representative metaphase plates of each lineage. Representative images of the large metacentric-chromosome markers (four, two and one for, respectively, lineages 3a and 3b, lineage 2c and lineage 2b) are also shown. Bayesian posterior probabilities are indicated in front of the nodes. Only Bayesian posterior probabilities > 0.90 are shown. The ancestral state reconstruction from the Maximum-Likelihood Markov model (Mk1) describing the (1) number of chromosomes and (2) the number of large metacentric chromosomes is also reported on the topology. Pie diagrams at each node indicate the proportion of the Maximum Likelihood supporting alternative reconstructed states. Bars define clades and lineages. The roman numbers after the specie’s names identify the haplotypes as in Table 1. Limnodirlus hoffmeisteri MTAC138 and L. hoffmeisteri MTAC 139 were considered as outgroups. Ch, chromosome number; Cl, clade; Li, lineage. Scale bar is 2 μm.