Figure 2

Examples of FISH with both 18S rDNA (green) and telomeric DNA (red) probes in spermatogonial metaphases (a, c, d, e, f, g) or diakinesis (b, h, i) as well as karyotypes arranged from these divisions (a’–h’) and silver staining in diakinesis (l) and diplotene (j, k, m) of chromosomes for the following Isophya species: I. thracica (a, a’, j), I. nervosa (b, b’), I. cf. armena (c, c’, k), I. brunneri (d, d’), I. rectipennis – Bulgarian population (e, e’) and Turkish population (f, f’), I. schneideri (g, g’, l), I. rizeensis (h, h’), and I. yaraligozi (i, i’, m). In the karyotypes, white arrowheads indicate the chromosomal location of rDNA clusters and an asterisk (*) marks differences in size between some homologous chromosomes (a’, c’, e’, f’, i’). Black arrowheads indicate the presence of one (j), two (k), three (l), or four (l) active NORs which coincide with 18S rDNA signals. Bar = 10 μm.