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Table 3 Behavior and ovarian activation in workers introduced to peer and experienced groups

From: Exploring the role of juvenile hormone and vitellogenin in reproduction and social behavior in bumble bees

 

Peer groups

Experienced groups

P value

 

Control

DMF

JH

Control

DMF

JH

 

Total HDT per group

70.8±10

48.9±15.1

52.1±16.8

50.75±12.2

54.2±12.1

37.3±7.3

p=0.56; p=0.341

n=10

n=10

n=7

n=8

n=10

n=9

HDT performed by IW

36.9±13.6

30.6±12.1

35.7±15

14.2±3.5

6.3±2.3

4.5±1.1

p=0.97; p=0.022

n=10

n=10

n=7

n=8

n=10

n=9

HDT received by IW

32.4±8.2

33.7±7.1

20.3±6.8

40.5±3.5

42.1±6.9

42±5.2

p=0.61; p=0.293

n=10

n=10

n=7

n=8

n=10

n=9

Percentage of HDT in which IW was involved

50.6±10.9%

35.8±15.8%

32.1±13.4%

26.25±6.1%

26.9±7.4%

17.4±3.9%

P=0.84; p=0.0264

n=10

n=10

n=7

n=8

n=10

n=9

Ovarian activation of IW (4 days)

0.14±0.01

0.12±0.01

0.17±0.01

0.17±0.02

0.16±0.02

0.13±0.02

p=0.13; p=0.685

n=29

n=28

n=26

n=8

n=10

n=9

Ovarian activation of IW (7 days)

2.24±0.2

-

-

1.47±0.23

-

-

t-test: t=2.46, p=0.01

(n=15)

  

(n=16)

  
  1. The introduced worker was assigned to one of three treatments: (1) 100ug JH-III diluted in 5 ul DMF applied to the thorax before introduction (2) 5ul DMF (3) untreated control. All workers were callow when introduced. Groups were composed of full sisters originated from the same colony (20 colonies in total) Statistical comparison was done using a nested ANOVA test. ‘IW’ refers to the introduced worker. HDT refers to the sum of humming, darting and attack behaviors received or performed by the introduced worker
  2. 1Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.7, p = 0.56, For group-type only: f(1,48) = 0.91, p = 0.34.
  3. 2Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.12, p = 0.97; For group-type only: f(1,48) = 5.4, p = 0.02.
  4. 3Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.67, p = 0.61; For group-type only: f(1,48) = 1.14, p = 0.29.
  5. 4Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.35, p = 0.84; For group-type only: f(1,48) = 5.27, p = 0.026. Data was transformed using arcsin.
  6. 5Nested ANOVA, treatment is nested in group-type: f(4,77) = 1.83, p = 0.13; For group-type only: f(1,77) = 0.16, p = 0.68.