Dating of origins of bee- and bird-pollination syndromes using ITS. Chronogram of Mirbelieae and Bossiaeeae derived from Maximum Likelihood analysis of ITS sequence data using PL rate smoothing. Parsimony trait reconstruction of bee- (blue) and bird- (red) pollination syndromes (no directional weighting) is shown on tree. Red bars show 95% confidence intervals of stem age of bird-pollination syndrome transitions and crown age of the earliest inferred bird-pollination syndrome transition (Leptosema). Posterior probabilities > 0.95 (PP) and bootstrap support > 70 (BS) are shown on stem and crown nodes of bird-pollination syndrome transitions. Where node support is lower than 0.95/70, PP and BS is also shown on the node above the transition. Inferred crown and stem age (95% highest probability density) of honeyeaters (Meliphagidae) is shaded grey.