Figure 2

Consensus and example sequence alignment of eEF1A and EFL. Consensus sequences aligned with example sequences for eEF1A and EFL were calculated at the 70% level using the Python program Consensus Finder [34]. Ruler coloring indicates the boundaries of the three domains. Shading behind residues shows conservation patterns: turquoise – strongly differentially conserved sites; blue – sites conserved in EFL but not eEF1A; green – sites conserved in eEF1A and not in EFL. Red boxes indicate the location of the nucleotide binding motifs of the G domain. Colored lines beneath the alignment indicate structural features as follows: orange – eEF1Bα interacting sites; blue – residues lining the amino-acyl moiety binding pocket; green – the extended loop of the helix-loop-helix on the ribosome binding surface.