Figure 5

Phylogeny of bacterial GluRS1 and GluRS2. Phylogenetic tree of bacterial GluRS1 and GluRS2 sequences (listed in Additional file 5) and annotated with bacterial phyla (abbreviations in Table 1). Experimentally determined glutamylation capacity of both GluRS1 and GluRS2 for selected bacterial species (H. pylori, A. ferrooxidans and T. maritima) with the two isoacceptors of tRNA^Glu (E1: ³⁴UUC and E2: ³⁴CUC) and tRNA^Gln (Q1: ³⁴UUG and Q2: ³⁴CUG), are projected in the respective clades, as productive or non-productive (empty/filled symbols). Branch support values < 0.7, calculated using aLRT statistics, are indicated.