Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli(family Hesperiidae) comprises three morphologically cryptic species

Bertrand, Claudia; Janzen, Daniel H; Hallwachs, Winnie; Burns, John M; Gibson, Joel F; Shokralla, Shadi; Hajibabaei, Mehrdad

doi:10.1186/1471-2148-14-153

Table 5 Results of 454-pyrosequencing ITS2 from the Urbanus bernikerni complex

From: Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli(family Hesperiidae) comprises three morphologically cryptic species

MID	Sample ID	Species	No. sequences	No. unique variants	No. variants (>2 seq)	No. sequences in variants (>2 seq)
22	07-SRNP-30614	Urbanus segnestami	133	117	7	16
24	07-SRNP-33232	Urbanus segnestami	147	136	6	11
26	08-SRNP-1439	Urbanus segnestami	207	172	15	35
27	08-SRNP-65935	Urbanus segnestami	66	46	8	20
28	08-SRNP-5848	Urbanus segnestami	174	150	11	24
29	08-SRNP-23800	Urbanus segnestami	142	117	9	25
30	08-SRNP-24278	Urbanus segnestami	183	137	18	46
52	07-SRNP-41187	Urbanus segnestami	120	91	13	29
53	06-SRNP-47304	Urbanus segnestami	80	63	8	17
54	06-SRNP-47955	Urbanus segnestami	170	117	23	53
55	06-SRNP-46570	Urbanus segnestami	143	115	13	28
56	08-SRNP-4727	Urbanus segnestami	43	36	3	7
31	06-SRNP-18276	Urbanus bernikerni	106	90	8	16
32	06-SRNP-46572	Urbanus bernikerni	151	120	24	71
33	07-SRNP-30615	Urbanus bernikerni	179	132	20	47
34	07-SRNP-30613	Urbanus bernikerni	233	189	17	44
37	07-SRNP-20318	Urbanus bernikerni	30	24	3	6
38	07-SRNP-57868	Urbanus bernikerni	56	50	3	6
39	07-SRNP-56547	Urbanus bernikerni	82	73	4	9
40	07-SRNP-56829	Urbanus bernikerni	322	266	23	56
57	07-SRNP-45071	Urbanus bernikerni	129	109	8	10
64	06-SRNP-47801	Urbanus bernikerni	240	166	30	74
65	06-SRNP-46460	Urbanus bernikerni	147	115	12	32
68	05-SRNP-1832	Urbanus bernikerni	4	4	0	0
69	01-SRNP-4475	Urbanus bernikerni	74	68	3	6
41	05-SRNP-41753	Urbanus ehakernae	164	125	17	39
42	05-SRNP-40545	Urbanus ehakernae	108	88	7	20
43	06-SRNP-65065	Urbanus ehakernae	11	11	0	0
45	07-SRNP-40761	Urbanus ehakernae	144	93	21	51
46	07-SRNP-33184	Urbanus ehakernae	169	118	18	51
47	07-SRNP-33183	Urbanus ehakernae	101	74	11	27
48	07-SRNP-33182	Urbanus ehakernae	117	80	17	37
49	08-SRNP-66028	Urbanus ehakernae	106	76	10	30
50	08-SRNP-65993	Urbanus ehakernae	156	126	13	30
70	06-SRNP-43129	Urbanus ehakernae	134	91	17	34
71	06-SRNP-46891	Urbanus ehakernae	190	156	11	34
72	06-SRNP-46887	Urbanus ehakernae	293	237	26	56
73	05-SRNP-42394	Urbanus ehakernae	112	87	11	25

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