Network diagram of micronucleate (black circles), and amicronucleate (red, new species; fuchsia, T. thermophila ) and shared (green) cox 1 haplotypes. Numbers indicate number of nucleotide changes within 663 base pair region of cox1 barcode. Black oval, T. thermophila. Green oval, haplotypes in New England states (CT, NH, MA, ME, VT). Yellow oval, haplotypes in eastern PA. Blue oval, haplotypes in western PA and Allegheny National Forest of PA. Green haplotype is that of type strain SB210 and is shared by both micronucleate and amicronucleate isolates.