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Table 3 Comparison of gene expression differences among sister populations and among individuals within populations

From: Parallel reduction in expression, but no loss of functional constraint, in two opsin paralogs within cave populations of Gammarus minus (Crustacea: Amphipoda)

Gene

Comparison

Source of variation

df

SS

MS

F

P

Ops1

SRC vs OC, uncorrected

Among Populations

1

148.894

148.894

188.553

5.600E-11

***

  

Among Individuals

38

30.007

0.790

5.184

0.000320

***

  

Trial

40

6.093

0.152

   
 

SRC vs OC, corrected for eye size

Among Populations

1

2277.056

2277.056

6.166

0.023105

*

  

Among Individuals

38

14033.710

369.308

4.123

0.001484

**

  

Trial

40

3583.039

89.576

   
 

US vs. HC, uncorrected

Among Populations

1

0.533

0.533

9.891

0.005599

**

  

Among Individuals

38

2.046

0.054

3.736

0.002749

**

  

Trial

40

2393.263

59.832

   
 

MDS vs. FRC, uncorrected

Among Populations

1

29.412

29.412

13.854

0.001560

**

  

Among Induviduals

38

80.673

2.123

16.813

2.307E-08

***

  

Trial

40

5.051

0.126

   
 

MDS vs. FRC, corrected for eye size

Among Populations

1

2182.124

2182.124

2.994

0.100701

ns

  

Among Individuals

38

27699.101

728.924

14.557

8.271E-08

***

Ops2

SRC vs. OC, uncorrected

Among Populations

1

39.946

39.946

47.245

0.000002

***

  

Among Individuals

38

32.129

0.846

6.270

0.000081

***

  

Trial

40

5.394

0.135

   
 

SRC vs. OC, corrected for eye size

Among Populations

1

8653.153

8653.153

15.578

0.000945

***

  

Among Individuals

38

551.633

14.517

24.123

8.650E-10

***

 

US vs. HC, uncorrected

Among Populations

1

126.647

126.647

8.724

0.008499

**

 

US vs. HC, corrected for eye size

Among Populations

1

74198.874

74198.87

6.09432

0.023806

*

  

Among Individuals

38

462653.1

12175.08

17.4017

1.69559E-08

***

  

Trial

40

27986.008

699.6502

   
 

MDS vs. FRC, uncorrected

Among Populations

1

48.912

48.912

21.098

0.000226

***

  

Among Individuals

38

88.099

2.318

17.337

1.753E-08

***

  

Trial

40

5.349

0.134

   
 

MDS vs. FRC, corrected for eye size

Among Populations

1

64.220

64.220

0.053

0.821281

ns

  

Among Individuals

38

46445.260

1222.244

7.781

0.000016

***

  

Trial

40

6283.156

157.079

   
  1. The statistical significance of differences in expression values were tested for using a nested ANOVA. Significance: *P < 0.05, **P < 0.01, ***P < 0.001.