Table 2 Details of primer pairs (restriction sites for Xba I and Bam HI, and fluorescent CAG and M13R binding sites underlined) used for the cloning of pike Elovl5 ORF in pYES2, qPCR analysis of tissue expression and for the genotyping of Atlantic salmon paralogous genes

E. lucius

elovl5 cDNA cloning

Elovl5

UNIE5OUTF

5’- ATGGATGGGTCCCAGAGA -3’

437 bp

55°C

JX272634^a

UNIE5OUTR

5’- AGTTCATAACGAACCACCAGAT -3’

UNIE5INNF

5’- TGGGGCCCAAGTACATGA -3’

388 bp

55°C

UNIE5INNR

5’- TGGACGAAGCTGTTAAGGG -3’

5’ RACE

Elovl5

PIKE5_5’F

5’- GATGGCAGAGAGCCCATAGT -3’

639 bp

55°C

JX272634^a

PIKE5_5’R

5’- CCACACAGCAGACACCATCT -3’

336 bp

55°C

3’ RACE

Elovl5

PIKE5_3’F

5’- AGATGGTGTCTGCTGTGTGG -3’

1118 bp

60°C

JX272634^a

PIKE5_3’R

5’- ATGCTCAACATCTGGTGGTTT -3’

915 bp

60°C

ORF cloning

Elovl5

PIKE5OUTF

5’- GCCCAGGTTCGCATCACCCAG -3’

1337 bp

60°C

JX272634^a

PIKE5OUTR

5’- ATTCCGGGGGTCATTTGAGATAGACG -3’

PIKE5ORF_F

5’- CCCGAGCTCGGATCCAAATGGAGACTTTTAATCAGAGACTTAACACC -3’

922 bp

60°C

PIKE5ORF_R

5’- GGGTCTAGACTCGAGCTTCAGTCCGCCCTCACTTTCCT -3’

qPCR

18S

qPCRp18SF

5’- TTCGAATGTCTGCCCTATCAAC -3’

128 bp

55°C

Contig2237^b

qPCRp18SR

5’- CCTTCCTTGGATGTGGTAGC -3’

Elovl5

qPCRpELO5F

5’- CCTTTGCACTGACCGTGATA -3’

195 bp

56°C

JX272634^a

qPCRpELO5R

5’- GCGTGTCCTGGCAGTAGAA -3’

Ef1-a1

qPCRpEF1F

5’- AAGATCGACCATCGTTCTGG -3’

209 bp

55°C

GH265867^a

qPCRpEF1R

5’- CTGGCAGCCTTCTTATCGAC -3’

S. salar

Genotyping

Elovl5a

MS_elovl5a_F

5’- ACAATTGCCATTTTTGCAGATAGC -3’

175-231 bp^c

58°C

AGKD01037727^a

MS_elovl5a_M13R

5’- GGATAACAATTTCACACAGGAGCCATTCTTGATCCGCTTAT -3’

Elovl5b

MS_elovl5b_CAG

5’- CAGTCGGGCGTCATCAAGCCCGATATGATATTACCGTATT -3’

172-181 bp^c

56°C

AGKD01030045^a

MS_elovl5b_R

5’- GTAAAATGGTGACTTTGGGTTCAG -3’