An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon

Table 1 Functional characterisation of Northern pike Elovl5 elongase, and its role in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA)

PUFA substrate	Product	Conversion (%)			Activity
		*E. lucius*	S. salar	S. salar
		Elovl5	Elovl5a	Elovl5b
18:3n-3*	20:3n-3	39	-	-	C18 → 20
	22:3n-3	4	-	-	C20 → 22
	Total	43	-	-
18:4n-3*	20:4n-3	67	56	58	C18 → 20
	22:4n-3	5	7	4	C20 → 22
	Total	72	63	62
20:5n-3*	22:5n-3	67	36	68	C20 → 22
	24:5n-3	5	1	1	C22 → 24
	Total	72	37	69
22:5n-3*	24:5n-3	4	1	1	C22 → 24
18:2n-6*	20:2n-6	26	-	-	C18 → 20
	22:2n-6	2	-	-	C20 → 22
	Total	28	-	-
18:3n-6*	20:3n-6	67	43	65	C18 → 20
	22:3n-6	7	5	6	C20 → 22
	Total	74	48	71
20:4n-6*	22:4n-6	35	23	48	C20 → 22
	24:4n-6	1	1	1	C22 → 24
	Total	36	24	49
22:4n-6*	24:4n-6	1	1	1	C22 → 24

Data are expressed as percentage of PUFA converted to elongated products at each step of the pathway and overall when yeast were transformed with pYES2-Elovl5 construct and incubated in presence of C18, C20 or C22 substrates. Atlantic salmon data extracted from [25].

ISSN: 2730-7182