Figure 4
From: Parallel evolution of senescence in annual fishes in response to extrinsic mortality
Expression of lipofuscin in captive individuals of N. furzeri species complex and N. rachovii species complex. (A) N. furzeri complex, expression of lipofuscin in the liver at age 21 weeks. (B) N. furzeri complex, expression of lipofuscin in the brain at age 21 weeks. (C) N. rachovii complex, expression of lipofuscin in the liver at age 21 weeks. (D) N. rachovii complex, expression of lipofuscin in the liver at age 21 weeks. FUR = N. furzeri, KUN = N. kuhntae, RAC = N. rachovii, PIE = N. pienaari. In all graphs, red points refer to populations from semi-arid habitats and blue point to populations from humid habitats. Lipofuscin is quantified as percentage of pixels in the image that are brighter than a fixed fluorescence threshold. *** = p < 0.001,Mann Whithney’s U-test. For pairwise comparisons see Additional file 6: Table S6, Additional file 7: Table S7, Additional file 8: Table S8 Additional file 9: Table S9.