Phylogenetic tree of the NAT gene family based on 77 vertebrate nucleotide sequences. Shown is the maximum likelihood tree built using PhyML (GTR + I + G) and rooted with the three fish species (Danio rerio, Gasterosteus aculeatus, Oryzias latipes). The bootstrap values of 1,000 replicates are shown as percentages at nodes. Bootstrap values are only shown for nodes with greater than 50% support. The clades of Afrotheria, Laurasiatheria, Lagomorpha, Rodentia and Primates are shown in aqua blue, purple, green, blue and red, respectively.