From: Convergent evolution of heat-inducibility during subfunctionalization of the Hsp70 gene family
Target | Primer/Probe | Nucleotide sequence | T m | Fragment length | PCR efficiency |
---|---|---|---|---|---|
Hsp70 CY-A | Hsp70cyA_rt_F | 5′-TCA GGA GCT GAT GAC AAA CCA A-3′ | 59°C | 74 bp | 90% |
 | Hsp70cyA_rt_R | 5′-ATC TCC TCT GGA TGG AAT TTC TTG-3′ | 59°C |  |  |
 | Hsp70cyA_MGB_VIC | 5′-TGT GGT TAA GTA TAA AGG TGA G-3′ | 67°C |  |  |
Hsp70 CY-B | Hsp70cyB_rt_F4 | 5′-TGC ATA TGG TGC TGC TGT TTA AG-3′ | 59°C | 133 bp | 89% |
 | Hsp70cyB_rt_R2 | 5′-TAA TAC ACT CAT TAC ACC ACC TGC AG-3′ | 59°C |  |  |
 | Hsp70cyB_MGB_VIC | 5′-CCA CTT TCA TTA GGA ATT-3′ | 68°C |  |  |
Hsp70 ER-A | Hsp70ERA_rt_F | 5′-GAA GTT CTC ACT AGA GCC AGA TTC G-3′ | 59°C | 76 bp | 93% |
 | Hsp70ERA_rt_R | 5′-CTG ATT ACA TGG GAC CAG TTG TCT-3′ | 58°C |  |  |
 | Hsp70ERA_MGB_VIC | 5′-ACT CAA TTC AGA TCT C-3′ | 68°C |  |  |
Hsp70 ER-B | Hsp70ERB_rt_F | 5′-GTT GAG AAG GGA ACC TAA TAA AAG GTC-3′ | 59°C | 98 bp | 92% |
 | Hsp70ERB_rt_R | 5′-GTC CCA AAT ATC CTT CAG CAA TCT-3′ | 59°C |  |  |
 | Hsp70ERB_MGB_VIC | 5′-CAG AGG AGA TTA GTG CCA TG-3′ | 68°C |  |  |
Hsp70 MT | Hsp70mt_rt_F | 5′-ACC AGA ATG CCA AAA GTC CAA-3′ | 58°C | 76 bp | 91% |
 | Hsp70mt_rt_R | 5′-CAT CTG GGT TGA CTG ACT TGT TG-3′ | 58°C |  |  |
 | Hsp70mt_MGB_VIC | 5′-AAG ATT TAT TCG ACA AAC CA-3′ | 67°C |  |  |
GAPDH | GAPDH_rt_F | 5′-GCT GCC AAG GCT GTT GGT-3′ | 58°C | 75 bp | 92% |
 | GAPDH_rt_R | 5′-TGT TGG AAC TCT GAA GGC CAT AC-3′ | 59°C |  |  |
 | GAPDH_MGB_FAM | 5′-TCC CAG AAA TCA AAG-3′ | 67°C |  |  |
EF-1a | EF1a_rtF2 | 5′-TTG ATG CCC CAG GAC ATA GAG-3′ | 59°C | 95 bp | 94% |
 | EF1a_rtR2 | 5′-TCC TGC TGG TGA GGC AAT C-3′ | 60°C |  |  |
 | EF1a_MGB_FAM | 5′-TAC AGG AAC ATC ATA AGC-3′ | 68°C |  |  |