Figure 2

Schematic representation of the collection of mutations obtained in the 60 experimentally evolved lineages. The first line represents the full TEV genome with the position of the eleven mature viral proteins within the ORF. The six other lines correspond each to one evolutionary history in Phase 1 and all the mutations obtained for this evolutionary history are represented. In green, new mutations; in blue mutations that got fixed (i.e. polymorphic at the end of Phase 1 and fixed for the mutant allele at the end of Phase 2); in red, reversion to the ancestral genotype; in orange, reverting sites (fixed for a mutant allele at the end of Phase 1 and polymorphic mutant/ancestral at the end of Phase 2) and in pink reversions from a polymorphic state (polymorphic mutant/ancestral at the end of Phase 1 and reverted to the ancestral state at the end of Phase 2). Circles piled above the line represent mutations at the exact same locus whereas when several mutations were close in the sequence, they are represented for clarity below the line.