Potential use of low-copy nuclear genes in DNA barcoding: a comparison with plastid genes in two Hawaiian plant radiations

Table 2 Variability and properties of the markers used in Cyrtandra

	psbA-trnH	Cyrt2	Cyrt4
Length, excluding primers	388-403	314	291
Number of variable sites (number of indels)	5 (2)	19	21
Percent of variable sites, excluding indels	1.29	6.05	7.21
Percent of “ghost” haplotypes	11.1	0	10
Ramification index	0.5	0.66	0.61

“Ghost” alleles are intermediate alleles that were not recovered in any of the accessions sampled. The ramification index (I) was calculated as follows: I = 1 – (longest distance between two haplotypes/total length of the network).

ISSN: 2730-7182