Figure 2

UV-visible dark absorption spectra of C. nuchalis SWS1 mutants. Dark spectra of (A) S90C, (B) C86F, (C) C86S, and (D) double mutant C86S/S90C, all recorded at pH 6.6. Insets show dark-minus-acid difference spectra. Estimated λ_max values indicated for each mutant.