Multilocus phylogeny and cryptic diversity in Asian shrew-like moles (Uropsilus, Talpidae): implications for taxonomy and conservation

Table 3 Primers used for PCR and sequencing

*Locus*	Primer name	Primer sequences (5′-3′)s	Sense/anti-sense	Annealing temperature (°C) *	Citation
12S	L613_hk1	GGCGGGCGAGCAAAGCACTGAAAATG	Sense	53-48	[40]
	H1478_hk1	TGATTGGTGGAGGGTGACGAGCGGTGTGT	Anti-sense
*CYT B*	L14724_hk3	GGACTTATGACATGAAAAATCATCGTTG	Sense	60-55	[40]
	H15915_hk3	GATTCCCCATTTCTGGTTTACAAGAC	Anti-sense
*RAG1*	F1705	GCTTTGATGGACATGGAAGAAGACAT	Sense	53-48	[52]
	R2951	GAGCCATCCCTCTCAATAATTTCAGG	Anti-sense
*RAG2*	RAG2-F220	GATTCCTGCTAYCTYCCTCCTCT	Sense	58-53	[52]
	RAG2-R995	CCCATGTTGCTTCCAAACCATA	Anti-sense
*ADORA3*	ADORA3-F	ACCCCCATGTTTGGCTGGAA	Sense	60-55	[50]
	ADORA3-R	GATAGGGTTCATCATGGAGTT	Anti-sense
*ATP7A*	ATP7A-F	TCCCTGGACAATCAAGAAGC	Sense	60-55	[50]
	ATP7A-R	AAGGTAGCATCAAATCCCATGT	Anti-sense
*BDNF*	BDNF-F	CATCCTTTTCCTTACTATGGTT	Sense	60-55	[50]
	BDNF-R	TTCCAGTGCCTTTTGTCTATG	Anti-sense
*BMI1*	BMI1-F	CATTGGGCCATAGTTTGTTAATCTCAA	Sense	60-55	[50]
	BMI1-R	CCAATATGGCATTGTACAACAAGC	Anti-sense
*CREM*	CREM-F	AGGAACTCAAGGCCCTCAAA	Sense	60-55	[50]
	CREM-R	GGGAGGACAAATGTCTTTCAA	Anti-sense
*PLCB4*	PLCB4-F	GTGAAATTGGAAGCCGAGAT	Sense	63-60	[50]
	PLCB4-R	CACCAAGCTCATTTACTTGTGA	Anti-sense

* Touchdown PCR programs were used for the amplification of each gene fragment. The higher annealing temperature was used in the first cycle and decreased by 0.5°C per cycles in the first 10 cycle. The lower annealing temperature was used for the next 25 cycles.

ISSN: 2730-7182