Figure 1

Selection on ϕ6 for fast and slow electrophoretic mobility. (A) Migration rate on agarose electrophoresis gel of ϕ6 virus lysate without manipulation. Rf is the migration of xylene cyanol dye relative to sampling points. (B) The progression of migration rate during the electrophoretic mobility selective regime. Four representative strains of ϕ6 derived strains along the slow (left panel) or fast (right panel) selection regime are shown. These represent ancestors of ϕ6-Slow2 and ϕ6-Fast2, respectively. Each line of a different color represents a strain from a given round of selection, i.e. passage number. (C) Density plot of relative mobility of ϕ6-Slow1 (SS), ϕ6-Fast1 (FF), and the single burst lysate of their cross at a multiplicity of infection of 10 (SSFF).