Figure 3

Relaxed molecular-clock phylogeny of the Tenthredinidae, and the distribution of various larval ecological and defensive traits within the group. The BEAST MCC tree is based on analysis of Dataset 2, which includes all sequenced tenthredinids as well as representatives from three non-blasticotomid families in Tenthredinoidea. Posterior probabilities (%) resulting from analyses in BEAST and MrBayes are given above and below branches, respectively (clades not present in the MrBayes tree are indicated by hyphens). Grey shaded bars show the 95% highest posterior density intervals for relative node ages for nodes with posterior probabilities exceeding 50%. Branch colors denote host plant classes of the sawfly species (see legend) and ancestral reconstructions based on maximum-likelihood optimization across 1,000 post-burnin trees (see Additional file 4A). In the table to the right of the tree, diet breadth, plant toxicity, and defensive traits (from left to right) are coded as shown in Table 1. (?) Unknown; (x) not applicable.