Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique

Table 1 Summary of analyzed material (31 localities for microsatellites and 36 for mtDNA)

Pop ID	Microsatellites		MtDNA		Lat	Long	Alt	Year
	N	IBD	N	Demo
1	20	LimpN	3	LimpN	−24.16	32.80	59	2010
2	24	LimpN	2	LimpN	−24.06	32.73	77	2008
8	21	LimpN	3	LimpN	−23.69	32.61	118	2008
13	25	LimpN	3	LimpN	−23.46	32.56	129	2008
23	21	Chefu	3	Chefu	−22.51	32.55	128	2008
34	22	Chefu	3	Chefu	−22.15	32.82	124	2010
43	21	Chefu	7	Chefu	−23.31	32.53	91	2008
50	25	LimpN	3	LimpN	−24.22	32.83	49	2008
53	26	LimpN	3	LimpN	−24.37	32.95	34	2008
55	28	LimpN	2	LimpN	−24.36	32.96	33	2008
119	23	LimpS	7	LimpS	−24.42	32.78	48	2010
120	34	LimpS	5	LimpS	−24.33	32.72	56	2008
121	36	LimpN	3	LimpN	−24.36	32.97	30	2008
123	-	-	4	LimpS	−24.65	32.44	88	2008
124	24	LimpS	6	LimpS (4)	−24.59	32.40	96	2008
207	20	LimpS	6	LimpS	−24.19	32.36	136	2009
215	20	Chefu	2	Chefu	−22.36	32.33	181	2009
218	-	-	5	Chefu	−22.31	32.62	149	2009
219	20	Chefu	3	Chefu	−22.24	32.51	176	2009
220	13	Chefu	3	Chefu	−22.02	32.80	146	2009
222	22	Chefu	3	Chefu	−21.87	32.80	158	2009
223	18	Chefu	5	Chefu	−21.66	33.10	149	2009
224	-	-	4	Chefu	−21.64	33.37	130	2009
225	11	Chefu	5	Chefu	−21.93	33.94	146	2009
322	23	Chefu	5	Chefu	−23.36	32.54	142	2010
323	20	Chefu	7	Chefu	−22.74	32.10	220	2010
325	-	-	6	-	−23.41	32.55	134	2010
326	24	LimpS	5	LimpS	−24.59	32.95	30	2010
328	-	-	1	-	−24.45	32.86	26	2010
406	20	-	6	-	−24.13	33.29	34	2011
414	20	Chefu	6	Chefu	−22.55	32.73	97	2011
418	23	Chefu	5	Chefu	−22.65	33.32	68	2011
421	21	Chefu	5	Chefu	−22.18	33.27	105	2011
422	24	Chefu	3	Chefu	−22.11	33.77	156	2011
423	22	Chefu	6	Chefu (5)	−22.09	33.92	120	2011
GRZ	2	-	1	-	−21.69	31.86	422	2008
Total	673		149	132

IBD group of populations for which isolation-by-distance was analysed, Demo group of populations used for analyses of historical demography (number of individual sequences used for analysis of historical demography is shown in parentheses if different from N), latitude (Lat), longitude (Long), altitude (Alt) and year of tissue collection (Year) of locations of particular populations (Pop ID). The populations 123, 124, and 328 geographically belong to the Incomati basin; however this group has never been identified as separate unit.

ISSN: 2730-7182