Figure 3

Identifying shifts in coevolutionary links between amino acids in the different bacterial clades. We have analysed the coevolution of GroES and GroEL in the different bacterial clades (colour coded circles) and compared involved residues with those identified across the entire bacterial phylogeny (stars). The distribution of the coevolving residues along GroEL is shown in the X-axis, while this distribution in GroES is shown in the Y-axis. The continuous bar at the very bottom of the figure represents the three different major domains of GroEL (Apical: blue, Intermediate: yellow and Equatorial: red). On top of the continuous bar we have also identified regions reported to be involved in folding-independet functions. These regions are color-coded as in [10]: 1, 3 and 11, orange: binding to mouse adipocytes; 2 and 12, binding to potato leafroll virus; 4, insecticidal neurotoxin; 5, Monocytes and T-cell activators; 6, Binding to primary mouse macrophages; 7 and 9, binding to lipopolysaccharides; 8, insecticidal toxin; 10 and 13, binding to cell surface of J774A.1 cells; 14, monocytes modulation activity.