Gene conversion yields novel gene combinations in paralogs of GOT1 in the copepod Tigriopus californicus

Table 3 Primer sequences and amplification conditions for GOT1 paralogs from T. californicus

Gene Region	Forward Primer (5′ to 3′)	Reverse Primer (5′ to 3′)	Size (bp)	Ann. Temp.
GOT1p1	AGAAGTTGGTCATTCATTCTTCATC	CTTATTGACGGCCTCATTGATGGA	1243	58°C
GOT1p2	ATATCCGTGCCCAAAAGCCTAC	CTTATTGACGGCCTCATTGATGGA	1249	58°C
GOT1Sr (5′end)	AGTATCCCAACATGTCTGCCTTCG	GCAATATAGATAATGAATAACGACACT	~1700¹	63˚C²
GOT1Sr (middle)	GAGTGTCGTTATTCATTATCTATATTGC	TCCTCAACCAGGTTTTAGAGTCA	1751 SD, 1722 AB, 2855 SCN	63°C
GOT1Sr (3′end)	CAATTGACTCTAAAACCTGGTTGAG	GGTTATTCTTGAATTTGTTGTGCTTCGT	~1400bp	63°C
GOT1Sd	CAGGAACAATGGAAGATCATAGCCA	GGTTATTCTTGAATTTGTTGTGCTTCGT	~1700bp
GOT1_6A	ATGGCCACAACCAAGTTTATTG	TGGTCTTTGATGGGGCCTTCGTTCG	~1600bp	55°C

¹ There are small size variations due to indels in these fragments.
² For the amplifications of the GOT1Sr gene fragments the Phusion kit (NEB) was used to amplify products.

ISSN: 2730-7182