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Table 2 DAVID gene ontology functional annotation enrichment analysis in mechanical simulators

From: Suboptimal evolutionary novel environments promote singular altered gravity responses of transcriptome during Drosophilametamorphosis

GBF ΔT ↓O2 G level Affected genes (limma raw(FDR)) Behaviour responses Metamorphosis/morphogenesis/organogenesis Stress responses GO clusters observed also in control conditions
  Reproductive/ mating/oviposition Sensorial/ hormonal/ odorant prot.   Abiotic (heat, light & hypoxia) Biotic (defense & immune sys.) Insect cuticle proteins Cytoesqueleton and cell adhesion Proteolysis Red-Ox states/energy Hemo/ metals/ions binding
RPM No No sim μg UP 218(0)   2.9×10 -4       3.5×10-2 2.5×10 -6  
    sim μg Down 117(0)    8.7×10-9     2×10-2    3.9×10-3
  Yes No sim μg UP 28(0)   1.1×10-3         
    sim μg Down 17(0)       1.9×10-3     
  Yes Yes sim μg UP 20(0)           
    sim μg Down 21(0)           
LDC No No 12g^ UP 85(0)         7×10-2 <10 -14 4.2×10-3
    12g^ Down 63(0)    1.7×10-3    1×10-3     
    6g^ UP 37(0)    2.4×10 -5        1.1×10-2
    6g^ Down 8(0)      1×10-2      
  Yes No 12g^ UP 873(185) 1.5×10-16 7.9×10-2 3×10 -4   1.9×10-8      
    12g^ Down 614(72)       4.1×10-12 8.9×10 -4   5.8×10 -5 5.7×10-3
    6g^ UP 288(0)     3.9×10-2      2×10-5 3.7×10-2
    6g^ Down 118(0)    2.9×10-3 2.9×10 -5 2.3×10-2   1.7×10-2    3.5×10-2
  No Yes 12g^ UP >103(1557) 4.1×10-10   5.9×10-15     1.2×10 -4    
    12g^ Down >103(1040)     4.7×10-3   8.4×10-22    5.1×10-7 3.4×10-3
    6g^ UP >103(1359) 1.3×10-3   <10 -15   7×10-2 3×10 -6 1.5×10-16    5.3×10 -4
    6g^ Down 912(720) 7.4×10-4 6.8×10-5   3.1×10 -6 1.7×10-2    1.9×10-9 5.4×10-11 5.8×10-7
  Yes Yes 12g^ UP 608(6) 9×10-11   3×10-14 5.1×10 -4 3.7×10-3   2.8×10-11    3.9×10-7
    12g Down 334(0)   5.1×10 -4        9.7×10 -5  
    6g UP 419(1256) 4.9×10-17 6.1×10-2 3×10-2   7.8×10-2   3.7×10-2 8×10-2 7×10 -6  
    6g Down 297(912)     9.6×10-12    2.5×10 -4   7.7×10-8 7×10 -4
  1. The number of up- or down-regulated probesets in each GBF facility (first column) and condition (column 2 to 4) has been calculated and is shown in the fifth column (filtering with a p-value limma < 0.001, except for the LDC conditions (p-value FDR < 0.05 due to the large number of probesets detected)). Relative enrichment analysis of these gene lists have been done, and the more frequent GO groups observed in experimental (columns 6 to 10), or 1g/environmental controls (columns 11 to 15) are included. Statistically meaningful p values are indicated in the table using italics and bold fonts to emphasize a greater level of enrichment in each particular GO group is observed. ^ indicates the presence of mechanical/rotational disturbances.