GEDI 15 × 13 clustering analysis based on the six altered gravity experiments. Colors in the main panels indicate the average log2ratios level of each cluster in this condition compared to 1g controls, following the vertical scale. The lower-left panel indicates cluster density (number of probesets per cluster) following the horizontal scale at the bottom. Comparison experiments in standard cultivation conditions in the lab are shown in the first column (constant temperature and open atmosphere, RPM/LDC). RPM/LDC transcriptome status under different environmental conditions have been shown in the second (ΔT i.e., cold step and open atmosphere), the third (optimal temperature but ↓O2, i.e. closed container nearly induced hypoxia) and the fourth column (double constrained experiment, ΔT and ↓O2). In the case of the magnet facilities, the experiments were performed in closed containers (↓O2). Each row represents an altered gravity level or an internal control (12g, 6g, rotational control, μgsim (RPM), 0g*, 1g*, 2g*, 1gOpen container control). * effective gravity is that of pure water (pupae were not levitating in free space, but attached to filter paper).