Table 4 Primers and annealing temperatures for qRT PCR

Achnenthes kuwaitensis

EFL (57)

5'-GTCACTTGATCTTCAAGCAG

5'-TGTCGGTGAAGAACTCCTTG

EF-1α (60)

5'-GAGGAGTTGACGAGAACACG

5'-TTGGAGACTCGAACTTCCAG

α-tubulin (60)

5'-TGGAGCCCTACAACTCCATC

5'-CACCAGGTTGGTCTGGAACTC

Asterionella glacialis

EFL (58)

5'-TATCTCTGAGCGTGAGATGAAG

5'-CTTGGTGTTGCACTGAATGG

EF-1α (54)

5'-TGAAGAACGAACTATGGAAG

5'-CCAAAGTGAAATATCGATTG

α-tubulin (58)

5'-ACATGGCATGCTGCCTCATG

5'-ATCCTCGAAAGAGCTTCTGC

Detonula confervacea

EFL (58)

5'-AGGAATCTCTGCTCGTGAG

5'-GAACTCCTTGGTGTTACACTG

EF-1α (58)

5'-GAAACCATCGACAAGTACG

5'-GAAACTTCCACAACGTGATATCG

α-tubulin (58)

5'-CAAATGCGCAGCGACAAGAC

5'-TTCCAGAACGGACCTCGTC

Thalassionema nitzschioides

EFL (56)

5'-AATCTCTGCTCGAGAGATGG

5'-TGTAGTGGTACTTGCCAGTG

EF-1α (56)

5'-CGTAGCCGAAAGCATAATAG

5'-CCAGACACTGATATCAATAG

α-tubulin (56)

5'-TTGTATGATGTCTGCCGTGG

5'-AAGCCTTCTCACGCGAAATA

Goniomonas sp.

EFL (60)

5’-CATCAAGGGTCTCAAGAAGGACAAC

5’-CAGTTGATGGCGGTCATCTTCATG

EF-1α (60)

5’-GTTCTCTGCTGGATACACTCCAGTG

5’-ACGCTATTCATGGAAGGCCTCAAC

α-tubulin (55)

5’-CATGTACCGTGGTGATGTCG

5’-CTGGACCTTGGCAAGATCAC