Alignments of partial amino acid sequences translated from the 27 Rhacophoridae MHC class I alleles. Dots indicate identity to Pome-UA*04 and signs “-” denote deletions. Sites in shaded columns represent the putative ABS deduced from structural information for human HLA molecules [47, 48]. Signs “*”, “**” refer to amino acids predicted to be under positive selection in the M8 model of PAML, with a posterior probability >95% and >99%, respectively. Signs “^” refer to positively selected sites identified by FEL and MEME.