Evolution at increased error rate leads to the coexistence of multiple adaptive pathways in an RNA virus

Table 5 Competition among different site-directed mutants of bacteriophage Qβ in the presence of AZC

Competitor viruses	Viruses present in the final population¹
	Transfer number 5	Transfer number 20
Qβ_wt + Qβ_A1746U	Qβ_A1746U	ND²
Qβ_wt + Qβ_A2982G	Qβ_A2982G	ND
Qβ_wt + Qβ_U3582C	Qβ_U3582C	ND
Qβ_A1746U + Qβ_A2982G	Qβ_A1746U	ND
Qβ_A1746U + Qβ_U3582C	Qβ_A1746U	ND
Qβ_A2982G + Qβ_U3582C	Qβ_A2982G +	Qβ_A2982G +
Qβ_A2982G + Qβ_U3582C	Qβ_U3582C	Qβ_U3582C
Qβ_A1746U + Qβ_{A1746U + A2982G}	Qβ_{A1746U + A2982G}	ND
Qβ_A1746U + Qβ_{A1746U + U3582C}	Qβ_{A1746U + U3582C}	ND
Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}	Qβ_{A1746U + A2982G} +	Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}
Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}	Qβ_{A1746U + U3582C}	Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}
Qβ_A1746U + Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}	(Qβ_A1746U)³ +	(Qβ_A1746U)³ +
Qβ_A1746U + Qβ_{A1746U + A2982G} + Qβ_{A1746U + U3582C}	Qβ_{A1746U + A2982G} +	Qβ_{A1746U + A2982G} +Qβ_{A1746U + U3582C}
	Qβ_{A1746U + U3582C}

¹Competitions were carried out as described in Methods. The viruses present in the populations obtained at the transfer indicated were identified through the determination of the consensus sequences at the nucleotide positions that distinguish the competitor viruses.
²ND means not determined.
³In this case, the determination of the consensus sequence do not allow distinguishing whether Qβ_A1746U is present in the populations analyzed or not. We show this virus in brackets to indicate this uncertainty.

ISSN: 2730-7182