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Table 4 Relative fitness of bacteriophage Qβ site-directed mutants

From: Evolution at increased error rate leads to the coexistence of multiple adaptive pathways in an RNA virus

Site-directed mutant

Relative fitness1

Relative fitness1

Dominant virus in competition with Qβwt2

 

+AZC

-AZC

+AZC

-AZC

A1746U

0.71 ± 0.02*

0.58 ± 0.03*

A1746U

wt

A2982G

1.24 ± 0.08*

0.89 ± 0.05*

A2982G

wt

U3582C

1.31 ± 0.03*

0.89 ± 0.06*

U3582C

wt

C3413G3

0.95 ± 0.04*

0.92 ± 0.07*

C3413G +wt (transfer 15)

C3413G +wt (transfer15)

G3945A3

1.1 ± 0.06*

1.02 ± 0.05

G3945A (transfer15)

G3945A (transfer15)

U3989C

0.79 ± 0.04*

0.88 ± 0.05*

wt

wt

  1. 1Relative fitness was evaluated with respect to the virus Qβwt either in the absence or the presence of AZC (60 μg/ml). The absolute fitness value estimated for Qβwt was 15.42 ± 0.50 in the absence of AZC and 9.68 ± 0.22 in its presence. The asterisk indicates that the difference between the fitness values of the site-directed mutant and the virus Qβwt was significant (p value < 0.05, Student’s t test for the difference of means).
  2. 2Competition between the site-directed mutants indicated and the virus Qβwt was carried out as described in Methods during 5 transfers either in the absence or the presence of AZC (60 μg/ml).
  3. 3Since no virus became dominant at transfer number 5, competitions carried out with viruses QβC3413G and QβG3945A were extended until transfer number 15, yielding the results shown in the table.