|
Site-directed mutant
|
Relative fitness1
|
Relative fitness1
|
Dominant virus in competition with Qβwt2
|
|---|
| |
+AZC
|
-AZC
|
+AZC
|
-AZC
|
|---|
|
QβA1746U
|
0.71 ± 0.02*
|
0.58 ± 0.03*
|
QβA1746U
|
Qβwt
|
|
QβA2982G
|
1.24 ± 0.08*
|
0.89 ± 0.05*
|
QβA2982G
|
Qβwt
|
|
QβU3582C
|
1.31 ± 0.03*
|
0.89 ± 0.06*
|
QβU3582C
|
Qβwt
|
|
QβC3413G3
|
0.95 ± 0.04*
|
0.92 ± 0.07*
|
QβC3413G + Qβwt (transfer 15)
|
QβC3413G + Qβwt (transfer15)
|
|
QβG3945A3
|
1.1 ± 0.06*
|
1.02 ± 0.05
|
QβG3945A (transfer15)
|
QβG3945A (transfer15)
|
|
QβU3989C
|
0.79 ± 0.04*
|
0.88 ± 0.05*
|
Qβwt
|
Qβwt
|
- 1Relative fitness was evaluated with respect to the virus Qβwt either in the absence or the presence of AZC (60 μg/ml). The absolute fitness value estimated for Qβwt was 15.42 ± 0.50 in the absence of AZC and 9.68 ± 0.22 in its presence. The asterisk indicates that the difference between the fitness values of the site-directed mutant and the virus Qβwt was significant (p value < 0.05, Student’s t test for the difference of means).
- 2Competition between the site-directed mutants indicated and the virus Qβwt was carried out as described in Methods during 5 transfers either in the absence or the presence of AZC (60 μg/ml).
- 3Since no virus became dominant at transfer number 5, competitions carried out with viruses QβC3413G and QβG3945A were extended until transfer number 15, yielding the results shown in the table.
