Figure 4

Expression of GmaPHO1 genes in response to salt and drought stresses. (a-d) Root expression of the Class I gene in response to PEG-6000 (grey column) or NaCl (black column). (e-h) Root expression of the Class II gene in response to PEG-6000 (grey column) or NaCl (black column). Genes are indicated in each graph. Empty column represents the untreated control. The 3-week old seedlings of ‘Nannong1138-2’ were treated with for 4 h and 8 h. Actin was used as an internal control. The experiments were performed based on three independent biological samples. Error bars=standard deviations. Star indicates the significance of gene expression variation under each treatment in comparison to the untreated control (*P < 0.05, **P < 0.01 and ***P < 0.001).