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Figure 2 | BMC Evolutionary Biology

Figure 2

From: The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene

Figure 2

Alignment of the marsupial ELP and eutherian CTI precursor proteins. The nucleotide sequences of the ELP/CTI mRNA transcripts of the following species were conceptually translated and aligned with ClustalW2: tammar [GenBank: JN191338; UniProtKB/Swiss-Prot: O62845 (mature protein)], brushtail possum [GenBank: U34208], fat-tailed dunnart (FT) [GenBank: JN191339], opossum [GenBank: JN191340], cow (Holstein-Friesian breed) [GenBank: JN191341] and dog [GenBank: JN191342]. The stripe-faced dunnart (SF) [GenBank: AC186006], koala [GenBank: JN191337], cat [GenBank: BK008083], pig [Ensembl: F1SD34_PIG (ENSSSCT00000008098)], dolphin [GenBank: BK008086], and panda [GenBank: BK008084] ELP/CTI genes were conceptually spliced based upon conserved splice sites and translated. Amino acid residues are numbered based upon the start (N-terminus) of the mature ELP/CTI peptides. Black shading indicates nucleotide residues common to at least 10 of the species and grey, the remainder that differ. The six conserved cysteine residues (C1-C6, C2-C4 and C3-C5), which form the three disulphide bonds and produce a globular protein are shaded red. Teal shading indicates amino acids common to marsupials and blue, those common to eutherians. The location of exons is indicated by arrows. The predicted signal peptides are boxed (blue). The BPTI KUNITZ 1 and 2 motifs are indicated (green and red bars respectively) and the putative trypsin interaction site from the KU motif (NCBI cd00109) is depicted by orange triangles. The putative P1 and P1' reactive site residues are shaded yellow and purple respectively. Italicised asparagine (N) residues indicate predicted sites of post-translational N-glycosylation. Conservation between groups of amino acids with strongly similar properties, i.e., scoring > 0.5 in the Gonnet PAM 250 matrix is indicated (:). Conservation between groups of amino acids with weakly similar properties (scoring < 0.5 in the Gonnet PAM 250 matrix) is also noted (.). Gaps within the alignment are indicated (−).

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