Reporter gene expression of autosomal and X-linked transgenes in A. gambiae. (A) Transmission and fluorescence (upper panels show RFP, lower panels show GFP) microphotographs of larvae heads and dissected testes taken from β2-eGFP 2 L, β2-eGFP-3R and β2-eGFP X mosquito lines. (B) Quantitative PCR analysis of the transcription activity of the neuronal 3xP3 and the testis specific β2-tubulin promoters. The bars show the ratios of quantitative RT-PCR signals detected in mosquito lines carrying autosomal integrations (β2-eGFP 2 L and β2-eGFP 3R) versus the X linked integration (β2-eGFP X) for the eGFP (green) and the DsRed (red) marker genes. (C) Expression activity of the α-tubulin promoter. Fluorescent microphotographs of six transgenic lines carrying single insertions of the α-tubulin-eGFP construct into either the X chromosome or one of the autosomes. The sites of chromosomal insertions are indicated on the top of the microphotographs. Red arrows in the photographs of the lower panel indicate testis.