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Table 2 McDonald-Kreitman test results at 54 loci between Daphnia pulex and an outgroup species

From: Immune genes undergo more adaptive evolution than non-immune system genes in Daphnia pulex

Classa

Gene

D. pulex

D. parvulaorD. arenata

Dnd

Dse

MK-test G-value (Yates correction)

p-value

  

Pnb

Psc

Pn

Ps

    

NI

Actin

0

4

0

0

0

8

NA

NA

NI

Caspase 1

0

12

0

0

0

8

NA

NA

NI

Caspase 2

28

18

4

9

1

0

NA

1.0000f

NI

Caspase 3

10

13

1

1

20

12

1.265

0.2608

NI

Caspase 4

22

10

7

7

10

4

0.134

0.7147

NI

Caspase 5

16

14

1

1

7

16

1.980

0.1594

NI

Caspase 6

27

8

1

1

12

7

0.441

0.5067

NI

Chitinase 1

12

27

0

0

5

7

0.121

0.7279

NI

Chitinase 9

18

43

0

1

2

2

0.088

0.7672

NI

Chitinase 15

5

17

11

5

6

8

0.004

0.9486

NI

Chitinase 16

3

12

7

3

9

19

0.095

0.7577

NI

Chitinase 17

48

71

3

1

13

18

0.012

0.9136

NI

Eukaryotic translation initiation factor 2γ

0

9

0

2

0

6

NA

NA

NI

Enolase

0

2

0

0

0

12

NA

NA

NI

Fumerate

2

13

0

2

3

28

0.073

0.7867

NI

Glutamine-oxaloacetic transaminase

0

15

1

1

3

17

0.130

0.7180

NI

Glyceraldehyde 3-phosphate dehydrogenase

0

4

0

0

0

9

NA

1.0000f

NI

Lactose dehydrogenase

4

18

0

0

0

13

NA

0.2735f

NI

Mannose phosphase isomerase

2

4

1

0

0

13

NA

0.0307 f

NI

Phosophoglucose isomerase

9

49

1

4

2

16

0.001

0.9765

NI

Phosophoglycerate mutase

4

56

2

0

3

17

0.061

0.8044

NI

Scavenger 5

1

30

3

11

5

27

0.258

0.6114

NI

Scavenger 6

7

21

5

7

6

16

0

0.9984

NI

Ultraspiracle

0

2

0

0

0

12

NA

NA

I

Caspase 8

55

27

1

0

33

11

0.466

0.4948

I

Dicer2

5

8

0

0

10

6

1.675

0.3593

I

Dorsal

0

12

0

0

0

8

NA

NA

I

Galectin 1

2

9

0

4

5

8

1.203

0.2727

I

Galectin 2

1

6

0

0

4

18

0.109

0.7412

I

Galectin 3

9

22

0

0

1

5

0.902

0.9022

I

Gemini

2

26

1

3

2

14

0.041

0.8386

I

Gram-negative binding protein 1

5

9

0

1

18

17

0.759

0.3835

I

Gram-negative binding protein 10

0

5

1

7

2

11

0.005

0.9411

I

Gram-negative binding protein 11

8

20

2

3

8

14

0.031

0.8605

I

Gram-negative binding protein 2

5

18

5

3

1

1

0.070

0.7918

I

Gram-negative binding protein 6

5

11

2

1

5

10

0.022

0.8818

I

Gram-negative binding protein 7

5

31

0

0

5

10

1.376

0.2408

I

IMD

10

12

1

2

5

10

0.112

0.7383

I

MyD88

3

11

3

2

13

21

0.035

0.8522

I

Nitric oxide synthase 1

1

14

0

1

2

4

0.821

0.3648

I

Nitric oxide synthase 2

8

6

2

1

5

15

1.076

0.2996

I

Pelle

0

6

1

2

3

9

0.058

0.8089

I

Prophenol Oxidase

13

15

2

9

0

0

NA

NA

I

Scavenger 2

24

18

7

2

16

22

2.107

0.1467

I

Scavenger 3

7

25

0

0

5

2

4.172

0.0411

I

Scavenger 4

10

42

1

0

5

12

0.134

0.7146

I

Stat

0

9

0

5

0

5

NA

NA

I

TEP 1

1

7

0

2

8

11

1.979

0.1596

I

TEP 2

4

18

0

3

3

21

0.996

0.3182

I

TEP 3

2

26

1

6

3

20

0.011

0.9155

I

Tequila

2

24

0

7

0

1

NA

1.0000f

I

Toll 1

1

6

1

0

18

7

3.771

0.0522

I

Toll 2

5

10

0

0

8

18

0.032

0.8581

I

Toll 3

1

14

0

3

0

5

NA

1.0000f

  1. Table 2 Legend: Significant P-values indicated in bold text. (a) NI= non-immune, I: immune, (b) Pn: number of nonsynonymous substitutions, (c) Ps: number of synonymous substitutions, (d) Dn: number of fixed non-synonymous differences between D. pulex and D. parvula or D. arenata, (e) Ds: number of fixed synonymous differences between D. pulex and D. parvula or D. arenata, (f) P-value based on Fisher's exact test (two-tailed), with Yate's correction.