Figure 7

Genomic evidence shows that perforin originated from a duplication of MPEG1, but the MACPF domain of perforin is more similar to C6. A. Genome scaffolds from the five fish species with assembled genomes that contain both MPEG1 and perforin genes are shown, along with the inferred scaffold of the last common ancestor (LCA) of Teleostei. B. Bayesian inference phylogenetic tree generated from an alignment of the MACPF domains of perforin, MPEG1 and C6 from human (Hsa), mouse (Mmu), anole lizard (Aca) and fugu (Tru). The tree is rooted at the midpoint. Node labels are posterior probabilities (italicised). Important branch lengths are labelled to indicate the degree of divergence between the three proteins.