Bayesian 50% majority-rule consensus tree of cloned ITS sequences. Bayesian tree inferred from cloned ITS sequences of P. marginata, P. allionii and P. latifolia. Posterior probability values are reported. The colors distinguish species and ploidy levels: P. marginata hexaploids = green. P. marginata dodecaploids = red. P. allionii = blu. P. latifolia = yellow. Accessions codes as in Table 4; clones are numbered progressively for each accession. Clade I: all sequences of P. latifolia; Clade II: all sequences from all1 population of P. allionii; Clade III: sequences from dodecaploid populations mar10, mar11, mar13, mar17 of P. marginata; Clade IV: sequences from the hexaploid mar6 population of P. marginata and all2 population of P. allionii. The two blue arrows indicate the ITS clones from individual all2 of P. allionii that form clades with ITS clones from P. marginata.