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Table 2 Stresses performed on female-sterile strains S1-B12 and S3-A10 and the corresponding wild-type strains S1 and S3

From: Asexual reproduction induces a rapid and permanent loss of sexual reproduction capacity in the rice fungal pathogen Magnaporthe oryzae: results of in vitroexperimental evolution assays

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Proportion of female-fertile replicates before treatment

Proportion of female-fertile replicates after treatments

Stress

S1

S3

S1-B12

S1-A10

S1

S3

S1-B12

S3-A10

-80Ā°C

(1 h)

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

-80Ā°C

(24 h)

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

Mycelium

mashing

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

single-spore

isolation

1/1

1/1

0/1

0/1

12/12

12/12

0/12

0/10

growth on plant

1/1

1/1

0/1

0/1

7/10

10/10

0/8

0/10

  1. The treatments were: one hour at -80Ā°C, 24 hours at -80Ā°C, mycelium mashing by ultrasound, single-spore isolation and growth on plants. The ratios (number of replicates in which the strain was female-fertile)/(number of replicates in which the strain was female-sterile) are given before and after the various treatments