A common, non-optimal phenotypic endpoint in experimental adaptations of bacteriophage lysis time

Table 3 ST-1 Genotypic evolution

Nucleotide	Gene	Change	Gene Function	End of phase I adaptation	ST-1_K-12
807	A	T- > C V50A	DNA replication	+*	+
1387	A	T- > C T210T	DNA replication	-	+
3869	F	G- > T V210F	Major coat protein	-	+
5540	H	A- > C D149G	Minor spike	+	+

All mutations from ST-1_K-12 were adaptive under conditions used for phase I of adaptation (R.H. Heineman, unpublished data)
*This mutation was only present in one of two sequenced isolates from this time point. The isolate with this additional change was used in all subsequent phenotypic assays

ISSN: 2730-7182