Table 2 Primers used for semi quantitative (RT-PCR) and real-time PCR (qPCR) of teleost-specific tlr s in Atlantic cod
Gene Name | qPCR primer (Forward and Reverse) (5′-3′) | Amplicon (bp) | RT-PCR/qPCR annealing (°C) | Efficiency (%) |
|---|---|---|---|---|
tlr21 | CGTTACAATCGCATCCTCTCAG GCTGCTCCACAACTCAGTCAAG | 177 | 58/60 | 110 |
tlr22a | GCAGGAAGTTCTGGAGACATTTA TCATTCACATTGGAGCACAAGTG | 186 | 58/60 | 98 |
tlr22b | GAGTTGGACTTTGGGACGAA ACATTCCTGACGGCACAAG | 128 | 58/60 | 125 |
tlr22c | TCAGTTCCCAATGCCGTAAG ACACAGTCCTTTAGAACCAAGACAC | 155 | 58/62 | 130 |
tlr22d | AGAGGAGGGTATGTTTGATGGC TGTTCGCTAAGTTCCGCAGTT | 152 | 58/62 | 116 |
tlr22e | CCAACCTCACAAGATTGAACCT GCAAGCGACAACCACTGATA | 120 | 58/60 | 115 |
tlr22f | CGCTTAGACCTGAGACACAACTT AATCCATCAAACATACCCTCCTC | 131 | 58/64 | 91 |
tlr22g | GCAGCAAACGAGATGTCCAC TCTCCCAGACGATACCATTCTC | 178 | 58/64 | 116 |
tlr22h | GCTTAGACCTGACACGCAACA AAGCCAGACGCAGTTCAATG | 159 | 58/62 | 130 |
tlr22i | GCATCGGTAGAGCCTATTCTGA GAAATTGGTCCGCTTATGAGA | 102 | 58/64 | 111 |
tlr22j | TGTGATTAGAGAACCAGTGATGCT TGTGTCTGCTTGTTTGTGATTACC | 129 | 58/62 | 92 |
tlr22k | TCCTACAATGGCAACTGGTCTAC CCCAGCCCTCGTCGTTTG | 129 | 58/60 | 88 |
tlr22l | CTCTTAGGCTGCTTAACACTTTAATC TGGATAGATAGATAACGCTGAGACG | 171 | 58/60 | 104 |
tlr23a | CCTTCGGCTACCACTTCCTG GCCTCGCTCGTCCTCCA | 188 | 58/62 | 110 |
tlr23b | GACTCCAATTTCCTCTGCTTCA GGTGCTGCTCATTATTCTTCCT | 163 | 58/64 | 94 |
luciferase | TCATTCTTCGCCAAAAGCACTCTG AGCCCATATCCTTGTCGTATCCC | 149 | 58/58 | 98 |
eef1a | CACTGAGGTGAAGTCCGTTG GGGGTCGTTCTTGCTGTCT | 142 | 58/58 | 110 |
ubi | GGCCGCAAAGATGCAGAT CTGGGCTCGACCTCAAGAGT | 69 | 69/60 | 92 |