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Table 3 Results of nested ANOVAs for chill-coma recovery time

From: Directional selection on cold tolerance does not constrain plastic capacity in a butterfly

A) Chill-coma recovery time

DF

MS

F

P

Selection Regime

1,6

24368.4

22.0

0.0032

Inbreeding Level

2,6

1183.4

1.1

0.4005

Replicate Line [Sel. & Inbr.]

6,4738

1204.3

7.7

<0.0001

Rearing Temperature

1,4738

127708.0

815.0

<0.0001

Adult Temperature

1,4738

115643.0

738.0

<0.0001

Sex

1,4738

<0.1

<0.1

0.9926

Selection Reg. * Inbreeding Level

2,6

192.6

0.2

0.8448

Selection Reg.* Rearing Temp.

1,4738

480.4

3.1

0.0800

Selection Reg. * Adult Temp.

1,4738

1327.2

8.5

0.0036

Selection Reg. * Sex

1,4738

21.2

0.1

0.7131

Inbreeding Level * Rearing Temp.

2,4738

797.1

5.1

0.0062

Inbreeding Level * Adult Temp.

2,4738

692.4

4.4

0.0121

Inbreeding Level * Sex

2,4738

254.0

1.6

0.1979

Rearing Temp. * Adult Temp.

1,4738

1.2

<0.1

0.9298

Rearing Temp. * Sex

1,4738

1.8

<0.1

0.9140

Adult Temp. * Sex

1,4738

1384.2

8.8

0.0030

Sel. Reg. * Inbreed. * Rear. Temp.

2,4738

910.95

5.8

0.0030

Sel. Reg. * Inbreed. * Adult Temp.

2,4738

527.1

3.4

0.0347

Inbreed. * Rear. Temp. * Adult Temp.

2,4738

504.9

3.2

0.0400

Rear. Temp. * Adult Temp. * Sex

1,4738

1121.7

7.2

0.0075

Error

4738

156.7

  

B) Animals reared at 20°C

DF

MS

F

P

Selection Regime

1,6

6782.9

18.8

0.0049

Inbreeding Level

2,6

213.8

0.6

0.5829

Replicate Line [Sel. & Inbr.]

6,1668

362.6

4.1

0.0004

Adult Temperature

1,1668

44483.1

501.1

<0.0001

Sex

1,1668

1.3

<0.1

0.9046

Selection Reg. * Adult Temp.

1,1668

1458.2

16.4

<0.0001

Error

1668

88.8

  

C) Animals reared at 27°C

DF

MS

F

P

Selection Regime

1,6

23033.8

15.3

0.0079

Inbreeding Level

2,6

2340.1

1.5

0.2859

Replicate Line [Sel. & Inbreed.]

6,3059

1506.7

7.9

<0.0001

Adult Temperature

1,3059

81814.6

427.7

<0.0001

Adult Feeding Regime

1,3059

5.6

<0.1

0.8644

Sex

1,3059

0.4

<0.1

0.9620

Selection Reg. * Inbreeding Level

2,6

959.2

0.6

0.5611

Selection Reg. * Adult Temp.

1,3059

321.2

1.7

0.1952

Selection Reg. * Adult Feed.

1,3059

66.0

0.3

0.5570

Selection Reg. * Sex

1,3059

38.0

0.2

0.6557

Inbreeding Level * Adult Temp.

2,305

1643.0

8.6

0.0002

Inbreeding Level * Adult Feed.

2,3059

211.2

1.1

0.3317

Inbreeding Level * Sex

2,3059

145.2

0.8

0.4682

Adult Temp. * Adult Feed.

1,3059

231.8

1.2

0.2711

Adult Temp. * Sex

1,3059

3459.7

18.1

<0.0001

Adult Feed. * Sex

1,3059

174.4

0.9

0.3397

Sel. Reg. * Inbreed. * Adult Temp.

2,3059

961.3

5.0

0.0066

Inbreed. * Adult Feed. * Sex

2,3059

1243.4

6.5

0.0015

Adult Temp. * Adult Feed. * Sex

1,3059

1362.7

7.1

0.0076

Error

3059

191.3

  
  1. Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, rearing temperature (20 vs. 27°C), adult temperature (20 vs. 27°C), and sex on chill-coma recovery time in Bicyclus anynana (A). In Tables B (animals reared at 20°C) and C (animals reared at 27°C) data were analyzed separately for each rearing temperature, as different feeding treatments were employed in animals reared at 27°C only. Consequently, the factor rearing temperature was dropped from both analyses, and the factor adult feeding regime (control vs. starvation) was added to Table C. For details see Material and Methods. Replicate line (random factor) was nested within selection regime and inbreeding level throughout. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.