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Table 3 PCR primers used to amplify CRI gene fragments. Y = C/T; R= A/G

From: Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss

PCR primers

Primer sequence (5′-3′)

Reference

AcePen_Glu-fora

GCT TTT CTC CAA GGT CTA CAG CTC

[33]

AcePen_Cyt1018-reva

GGG TGT TCT ACT GGT TGG CTG CC

[33]

Penpan_CRI-spec-180rev

CRY YRT YYA CAT TAA GTG A

This study

PenpanGui_CRI-spec-182rev

GTC ATT ATC TAC ATT AAG TAA G

This study

Penmanman_CRI-spec-185rev

TTA ATA TGT CGT TGT TTA CAT G

This study

Penaff_CRI-spec-186rev

GTA TGT CAT TGC TTG CAT TGA GTA G

This study

Acewal_CRI-spec-180rev

CAT TRT CTG CAT TTA AGC GT

This study

Penpan_CRI-spec-128for

ACG ACT AGT TAT TAA TGC T

This study

Penmanman_CRI-spec-115for

CAT AAG GTA ATG CTC TAT ACA ATT

This study

Penaff_CRI-spec-124for

CTA TAT GAT TAA CTA TTA ATG CTC

This study

Acewal_CRI-spec-130for

GAT TGA CTG TCA ATG TTT GT

This study

AcePen_644reva

AAG GGA ACC AAC AGT GCC AAA C

This study

AcePen_426reva

GTT GCT GAT TTC TCG TGA GG

This study

  1. a primers with two annealing sites due to the duplication of a large fragment of the hornbills’ mt genome [33].