Figure 2

C. elegans signaling pathways involved in developmental modulation of longevity. Survival of C. elegans mutants to E. coli 536 pathogen following development on strains 536 (536/536) or OP50 (OP50/536) and life through OP50 control (OP50/OP50). (A &B) C. elegans daf-16 mutants allele mu86 (536/536 n = 80, OP50/536 n = 80, and OP50/OP50 n = 80, independent repeats N = 4) and mgDf50 (536/536 n = 65, OP50/536 n = 56, and OP50/OP50 n = 61, N = 3), (C) C. elegans dbl-1 mutant (536/536 n = 127, OP50/536 n = 124, and OP50/OP50 n = 88, N = 4), and (D) C. elegans pmk-1 mutant (536/536 n = 80, OP50/536 n = 78, and OP50/OP50 n = 77, N = 8). No differential survival of daf-16 and dbl-1 nematodes to E. coli 536 based on developmental conditions was observed, while a small but statistically significant difference in survival to E. coli 536 strain was observed for C. elegans pmk-1 mutant depending on developmental conditions. Graphed: ns: not significant, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001.