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Table 2 Characterized carbohydrate-active enzymes of family GH5 not yet classified into subfamilies

From: Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5)

Description

EC

Assay

Organism

Accessions

Modular structure

Taxonomic class

β-mannanase A (ManA;CelA)

3.2.1.78

 

Caldanaerobius polysaccharolyticus KM-THCJ

AAD09354

GH5 CBM16 CBM16

B-Firmicutes_Clostridia

endo-β-1,4-glucanase D (CelD; CelCCD; EGCCD; Ccel_0840)

3.2.1.4

 

Clostridium cellulolyticum H10 [B]

BAA14354 ACL75216

GH5 CBM11 DOC

B-Firmicutes_Clostridia

endo-β-1,4-glucanase/b-1,3:1,4-glucanase H (CelH)

3.2.1.4 3.2.1.73

 

Clostridium thermocellum NCIB 10682

AAA23225

GH26 GH5 CBM11 DOC

B-Firmicutes_Clostridia

cellulase (EBI-244)

3.2.1.4

 

Desulfurococcaceae archaeon EBI-244

AEB53062

GH5

A-Crenarchaeota

endo-β-1,4-glucanase 3 (Cel3;Cel-3; Eg3; Fisuc_2230; FSU_2772)

3.2.1.4

 

Fibrobacter succinogenes subsp. succinogenes S85

AAA24893 ACX75816 ADL25000

GH5

B-Fibrobacteres_Acidobacteria group

Fisuc_2933/FSU_0196

 

AGM

Fibrobacter succinogenes subsp. succinogenes S85

ACX76513 ADL26912

GH5 CBM4

B-Fibrobacteres_Acidobacteria group

Fisuc_1523/FSU_2005

 

MUC

Fibrobacter succinogenes subsp. succinogenes S85

ACX75120 ADL26743

GH5

B-Fibrobacteres_Acidobacteria group

endoglucanase (CelA; lpg1918)

3.2.1.4

AHEC

Legionella pneumophila subsp. pneumophila str. Philadelphia 1

AAU27988

GH5

B-Gammaproteobacteria

endo-β-1,4-glucanase 5B (Sde_2490)

3.2.1.4

 

Saccharophagus degradans 2-40

ABD81750

CBM6 GH5

B-Gammaproteobacteria

endo-β-1,4-glucanase 5E (Sde_2929)

3.2.1.4

 

Saccharophagus degradans 2-40

ABD82186

CBM6 CBM6 GH5

B-Gammaproteobacteria

endo-β-1,6-glucanase (Exg3; SPBC2D10.05)

3.2.1.75

 

Schizosaccharomyces pombe 972 h-

CAA21163 NP_596224

GH5

E-Fungi

endo-β-1,4-glucanase (Cel5G)

3.2.1.4

 

uncultured bacterium

ADD71777

GH5

B-environmental samples

SARM_0034/694713_55880/TW39

 

LIC CMC

uncultured organism

ADX05705

GH5

U-unclassified sequences

SARM_0047/1057205_158590/TW-15

 

CMC

uncultured organism

ADX05718

GH5

U-unclassified sequences

SARM_0086/0_06533/TW-18

 

PCW

uncultured organism

ADX05761

GH5

U-unclassified sequences

β-glucanase (RR.06; RR.06-1; BglC)

 

BBG Cel5 LIC

unidentified microorganism

CAJ19140

GH5

U-unclassified sequences

β-glucanase (RR.10; RR.10-1)

 

BBG

unidentified microorganism

CAJ19146

 

U-unclassified sequences

  1. The active enzymes are tagged by their Enzyme Classification (EC) number (see Table 1) or by the significant positively assayed substrates that are: AEHC - AZCL-HE cellulose; AGM - AZCL-galactomannan; BBG – barley β-glucan; Cel5 – cellopentose; CMC - carboxymethyl cellulose; LIC –lichenan; MUC - 4-methylumbelliferyl-β-D-cellobioside; PCW – plant cell wall.