Investigating the molecular basis of local adaptation to thermal stress: population differences in gene expression across the transcriptome of the copepod Tigriopus californicus

Table 4 Comparison of RNA-seq and qPCR measurements of fold change in gene expression in selected genes

	Gene	SD fold change		SC fold change		SD accession	SC accession
		RNA-seq	qPCR	RNA-seq	qPCR
Technical replicates	Ubiquitin	0.9	0.1	0.6	0.1	JW512238	JW538961
	hspbeta-1 #2	22.9	20.6	4.5	2.1	JW506232	JW525782
	hspbeta-1 #3	24.6	13.6	4.2	4.7	JW506234	JW525781
	hspbeta-1 #4	105.2	109.5	4.7	2.3	JW506233	JW525780
	hspbeta-1 #5	4.9	2.8	2.1	1.8	JW519143	JW525778
	hsp20 #1	38.2	47	3	7.1	JW519178	JW525783
	hsp70 #2	224	35.9	7.8	2.4	JW519350	JW526654
	hsp70 #3	0.7	1.1	1.8	4.5	JW506218	JW525763
	hsp70 #4	0.9	0.7	1.3	1.9	JW506217	JW525759
	hsp70 #5	13	8.2	3.8	4.2	JW506905	JW526653
	hsp70 #15	6.1	4.4	2.6	2.8	JW502097	JW521081
	hsp70 #16	2.7	1.5	1.3	1.5	JW506226	JW526652
	hsp70 #17	1	0.7	1.8	2.2	JW506216	JW525758
	DNAJ #1	1.1	0.5	2.1	2.5	JW503763	JW522979
	DNAJ #2	1.2	1.4	1.2	2	JW504839	JW524198
	DNAJ #3	1.2	0.7	1	4.2	JW503761	JW522978
	DNAJ #4	1.1	0.6	0.5	0.8	JW503762	JW522980
	hsp90 #1	5.8	1.3	3.3	7.5	JW506231	JW525777
Biological replicates	hspbeta-1 #3	24.6	30.7	4.2	7.3	JW506234	JW525781
	hsp20 #1	38.2	24.2	3	3	JW519178	JW525783
	hsp70 #1	2.5	11	1.3	6	JW519142	JW525772
	hsp70 #2	224	95.1	7.8	19.8	JW519350	JW526654
	hsp70 #3	0.7	1	1.8	0.6	JW506218	JW525763
	hsp70 #4	0.9	0.8	1.3	0.8	JW506217	JW525759
	hsp70 #5	13	15	3.8	5.2	JW506905	JW526653
	hsp70 #15	6.1	5.2	2.6	1	JW502097	JW521081
	hsp70 #16	2.7	1.2	1.3	1.4	JW506226	JW526652
	hsp70 #17	1	0.8	1.8	0.8	JW506216	JW525758
	DNAJ #1	1.1	0.9	2.1	1	JW503763	JW522979
	DNAJ #2	1.2	1	1.2	1	JW504839	JW524198
	DNAJ #3	1.2	4.2	1	2.8	JW503761	JW522978
	DNAJ #4	1.1	1.3	0.5	1.5	JW503762	JW522980

ISSN: 2730-7182