Figure 2
1stpart of a series of horizontal vibratome sections of the brain of Speleonectes tulumensis . (A-D) Dorsal to ventral series of vibratome sections (50 μm) through the brain of Speleonectes tulumensis triple labeled for serotonin immunoreactivity (5HT-IR, green), acetylated α-tubulin immunoreactivity (TUB-IR, magenta) and nuclear marker (NUC, blue). Maximum projections consisting of 32 to 44 confocal laser-scans per slice. Arrow in (C) indicates a neurite bundle that comprises the axons from the olfactory receptor neurons. Abbreviations: capital letters A, B, C, E, G: soma clusters; A1Nv: antenna 1 nerve; HE: hemiellipsoid body; LAN2: lateral antenna 1 neuropil 2; OGT: olfactory globular tract; ON: olfactory neuropil; PCN: protocerebral neuropil; sA1, sB1, sB2: 5HT-ir cell groups; x: chiasm of the OGT.